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PD-L2 Antibody
SKU: orb2642798
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FACS, IF, WB |
|---|---|
| Dilution range | Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | CDLA273-1 |
| Immunogen | A recombinant fragment of human PD-L2 protein within amino acids 27-220 was used as the immunogen for the PD-L2 antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Western blot testing of 1) human Raji and 2) mouse RAW264.7 cell lysate with PD-L2 antibody (clone CDLA273-1). Expected molecular weight: 31-60 kDa depending on glycosylation level.
Flow cytometry testing of human Jurkat cells with PD-L2 antibody (clone CDLA273-1); Red=isotype control, Blue= PD-L2 antibody.
Immunofluorescent staining of human Jurkat cells with PD-L2 antibody (green, clone CDLA273-1) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
PD-L2 Antibody (orb2642798)
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