Pdcd4 phospho S457 Antibody

Biorbyt Antibody orb344433 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Western blot using Biorbyt's Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody against recombinant PDCD4 protein. Membrane was blocked in 1% BSA-TBS-T for 30 min RT and probed with 1°Ab Ms-A-Pdcd4pS457 1:1000 (o/n 4°C in 1% BSA-TBS-T) followed by 2°Ab Peroxidase Conjugated Rabbit anti-Ms CUST10M at 1:40000 in orb348637 30 min RT. Bands at ~62 kD and ~32 kD were detected.

Western blot using Biorbyt's Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kDa) in NIH-3T3 cells (p/n orb348723) after 5 min treatment with 30 ng/mL PDGF [lane 2]. No reactivity is seen for (non-phosphorylated) in unstimulated NIH 3T3 cells (p/n orb348714) [lane 1]. The membrane was probed with the primary antibody at a 1:2000 dilution, overnight at 4°C. For detection HRP conjugated Rb-a-Mouse IgG (p/n orb347544) was used at a 1:20000 dilution in blocking buffer (p/n orb348637) for 1 h at 4°C.