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PGAM5 Antibody
SKU: orb1239864
Featured
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 7
| Tested Applications | ELISA, IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Monkey, Primate |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-PGAM5 antibody (orb1239864) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human PGAM5. The immunogen is located within the last 50 amino acids of PGAM5. |
| Target | PGAM5 |
| Molecular Weight | Predicted: 32kD Observed: 32 kD |
| Purification | PGAM5 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PGAM5 Antibody is supplied in PBS containing 0.02% sodium azide |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Serine/threonine-protein phosphatase PGAM5 mitochondrial, Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5
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Western Blot Validation in Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of PGAM5 in Human Liver. Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver labeling PGAM5 with orb1239864 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PGAM5 in Mouse Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with orb1239864 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PGAM5 in Rat Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with orb1239864 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
PGAM5 Antibody (orb1239864)
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