Phospho-Histone H3 (Thr32) Rabbit Polyclonal Antibody

SKU: orb1172881

Description

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target	H3C1
Purification	Antigen affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

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Phospho-Histone H3 (Thr32) Rabbit Polyclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + Nocodazole (100 ng/ml, 18hr) + Calyculin A (100nM, 1hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.

Quick Database Links

Gene Symbol

H3C1

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Protocol Information

Western Blot (IB, immunoblot)

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