Phospho-PDHE1A(S232) Antibody

SKU: orb1931033

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	DOT, WB
Dilution Range	WB: 1:500, WB: 1:5000, DB: 1:500
Reactivity	Human
Predicted Reactivity	Mouse, Porcine, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	RB29473
Target	This PDHE1A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S232 of human PDHE1A.
Molecular Weight	43296 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I, PDHA1, PHE1A

Quality Guarantee

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Dot blot analysis of anti-mPDHE1A-S232 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Western blot analysis of lysates from C6 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A (S232) Antibody (upper) or Tubulin (lower).

Western blot analysis of lysates from 293 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A (S232) Antibody (upper) or Tubulin (lower).

Quick Database Links

Gene Symbol

This PDHE1A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S232 of human PDHE1A.

UniProt

P08559

RefSeq (Protein):NP_001166927.1, NP_000275.1, NP_001166926.1, NP_001166925.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001166927.1, NP_000275.1, NP_001166926.1, NP_001166925.1

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Protocol Information

Western Blot (IB, immunoblot)

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DOT

Dot Blot

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