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PKM2 Antibody
SKU: orb395450
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, IP, WB |
|---|---|
| Dilution range | WB: 1:1000-1:4000; IP: 1:200-1:1000; IHC: 1:100-1:400; IF: 1:10-1:100 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | PKM2 pyruvate kinase, muscle |
| Target | PKM2 |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Disclaimer | For research use only |
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MCF7 cells were subjected to SDS PAGE followed by western blot with orb395450 (PKM2-specific antibody) at dilution of 1:1500.
Immunofluorescent analysis of MCF-7 cells, using orb395450 (PKM2-specific Antibody) at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PKM2-specific (IP: orb395450, 4 ug; Detection: orb395450 1:300) with MCF-7 cells lysate 3250ug.
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using orb395450 (PKM2-specific Antibody) at dilution of 1:200.
Quick Database Links
Gene Symbol
PKM2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
PKM2 Antibody (orb395450)
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