PLOD1 Antibody (N-term)

All lanes: Anti-PLOD1 Antibody (N-term) at 1:1000-1:2000 dilution. Lane 1: MCF-7 whole cell lysates. Lane 2: K562 whole cell lysates. Lane 3: U-2OS whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PLOD1 Antibody (N-term) at 1:2000 dilution. Lane 1: A431 whole cell lysates. Lane 2: MCF-7 whole cell lysates. Lane 3: U-2OS whole cell lysates. Lane 4: U-87 MG whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes:PLOD1 Antibody (N-term) at 1:1000 dilution. Lane 1: U-87 MG whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Observed band size:90kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining PLOD1 in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Overlay histogram showing U-87 MG cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

PLOD1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PLOD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

PLOD1 Antibody (N-term) western blot analysis in U251 cell line lysates (35 ug/lane). This demonstrates the PLOD1 antibody detected the PLOD1 protein (arrow).