polo-like kinase 3 Antibody

SKU: orb1248448

Description

Research Area

Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Reactivity	Human
Application Notes	Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell line HeLa (calculated MW of 71.6kDa according to NP_004064.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows vesicular staining in the cystoplasm of collective tubules. Recommended concentration, 5-10ug/ml. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	The immunogen for this antibody is: PDRLPISSCVTVPD
Target	polo-like kinase 3
Molecular Weight	Approx 70kDa
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Disclaimer	For research use only

Alternative Names

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PLK3, polo-like kinase 3, CNK, FNK, PRK, FGF-inducible kinase, PLK-3, cytokine-inducible kinase, cytokine-inducible serine/threonine-protein kinase, proliferation-related kinase, serine/threonine-protein kinase PLK3

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orb1248448 (2 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248448 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

polo-like kinase 3

UniProt

Q9H4B4

RefSeq (Protein):NP_004064.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_004064.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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