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PRDM14 Antibody
SKU: orb1257447
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ChIP, IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-571 of human PRDM14 (AAH52311.1). |
| Target | PRDM14 |
| Molecular Weight | Observed: 64kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PRDM14, PFM11
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Western blot analysis of extracts of rat testis, using PRDM14 antibody (orb1257447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using PRDM14 antibody (orb1257447) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using PRDM14 antibody (orb1257447) at dilution of 1:100. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using PRDM14 antibody (orb1257447) and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Quick Database Links
Gene Symbol
PRDM14
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
PRDM14 Antibody (orb1257447)
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