Prolactin Receptor Antibody Cocktail

SKU: orb385758

Description

It recognizes a protein of 70kDa, identified as prolactin receptor. Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor), which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription.

Research Area

Epigenetics

Images & Validation

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Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	The optimal dilution of the Prolactin Receptor antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1 + IgG1
Clone No.	B6.2 + PRLR742
Immunogen	Semi-purified human prolactin receptor (B6.2) and recombinant human protein (PRLR742) were used as the immunogens for this Prolactin Receptor antibody cocktail.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Prolactin Receptor antibody (B6.2 + PRLR742).

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UniProt

P16471

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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