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PTTG2 Antibody (Center)
SKU: orb1934495
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Dilution range | IF: 1:10~50, WB: 1:2000, WB: 1:1000 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB35901 |
| Target | This PTTG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the Central region of human PTTG2. |
| Molecular Weight | 22302 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−Securin-2, Pituitary tumor-transforming gene 2 protein, PTTG2
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PTTG2 Antibody (Center) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the PTTG2 antibody detected the PTTG2 protein (arrow).
Anti-PTTG2 Antibody (Center) at 1:2000 dilution + human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent confocal image of MCF-7 cell stained with PTTG2 Antibody (Center). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTTG2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). PTTG2 immunoreactivity is localized to Cytoplasm significantly.
Quick Database Links
Gene Symbol
This PTTG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the Central region of human PTTG2.
UniProt
RefSeq (Protein):NP_006598.2
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_006598.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
PTTG2 Antibody (Center) (orb1934495)
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