You have no items in your shopping cart.
Rab5 Antibody
SKU: orb1822428
Description
Research Area
Cell Biology, Disease Biomarkers, Neuroscience, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution range | WB (1:1000), IHC (1:100), ICC/IF (1:80); optimal dilutions for assays should be determined by the user. |
| Reactivity | Bovine, Human, Monkey, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues |
| Target | Rab5 |
| Molecular Weight | 26kDa |
| Purification | Protein A Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Rab 5A, RAS associated protein RAB5A, Ras related protein Rab 5 A
Similar Products
−- Item 1 of 8
- Item 1 of 7
- Item 1 of 6
PP2A-alpha/PPP2CA Antibody (monoclonal, 3B6) [orb654389]
FC, ICC, IF, IHC, WB
Human, Monkey, Mouse, Rat
Mouse
Monoclonal
Unconjugated
100 μg - Item 1 of 6
SUN2 Recombinant Rabbit Monoclonal Antibody [orb1499408]
ICC, IF, IHC-Fr, IHC-P, WB
Human, Mouse, Rat
Rabbit
Recombinant
Unconjugated
100 μl, 50 μl - Item 1 of 4
Rab5 Antibody (C-term) [orb1929681]
FC, IF, IHC-P, WB
Human
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab5 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:80 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody. (C) Composite.
Western blot analysis of Human Cell line lysates showing detection of Rab5 protein using Rabbit Anti-Rab5 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Rabbit Anti-Rab5 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab5 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:80 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody. (C) Composite.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_004153.2 |
|---|
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
Rab5 Antibody (orb1822428)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 2-3 weeks