Rabbit anti-Lamin-A Antibody

SKU: orb1524848

Description

Lamin-A Antibody

Images & Validation

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Item 1 of 5

Tested Applications	ICC, IHC, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:50 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	Between 425 and 475
Target	Lamin-A
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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prelamin-A/C; LDP1; LGMD1B; LMN1; LMNC; LMNL1; lamin; mandibuloacral dysplasia type A; lamin A/C-like 1; PRO1; renal carcinoma antigen NY-REN-32; MADA; CDCD1; LFP; 70 kDa lamin; IDC; CDDC; CMD1A; CMT2B1; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; EMD2

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Detection of human Lamin-A by western blot. Samples: Whole cell lysate (15 and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-Lamin-A antibody orb1524848 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Lamin-A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Lamin-A antibody orb1524848 used for IP at 6 µg/mg lysate.

Detection of mouse Lamin-A by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-Lamin-A (Cat. No. orb1524848) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human Lamin-A by immunohistochemistry.Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Lamin-A (Cat. No. orb1524848) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human Lamin-A by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Lamin-A (Cat. No. orb1524848) used at a dilution of 1: 200 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100.

Quick Database Links

Gene Symbol

Lamin-A

UniProt

P02545

RefSeq (Protein):NP_733821.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_733821.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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