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Rabbit anti-Lamin-A Antibody
SKU: orb1524849
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ICC, IHC, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:50 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 425 and 475 |
| Target | Lamin-A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−prelamin-A/C; LDP1; LGMD1B; LMN1; LMNC; LMNL1; lamin; mandibuloacral dysplasia type A; lamin A/C-like 1; PRO1; renal carcinoma antigen NY-REN-32; MADA; CDCD1; LFP; 70 kDa lamin; IDC; CDDC; CMD1A; CMT2B1; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; EMD2
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Detection of human Lamin-A by western blot. Samples: Whole cell lysate (15 and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-Lamin-A antibody orb1524849 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Lamin-A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Lamin-A antibody orb1524849 used for IP at 6 µg/mg lysate.
Detection of human Lamin-A by immunohistochemistry.Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Lamin-A (Cat. No. orb1524849) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
Detection of mouse Lamin-A by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-Lamin-A (Cat. No. orb1524849) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
Detection of human Lamin-A by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Lamin-A (Cat. No. orb1524849) used at a dilution of 1: 200 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_733821.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
Rabbit anti-Lamin-A Antibody (orb1524849)
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