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Rabbit RALY Antibody

SKU: orb1527278

Description

RALY Antibody

Images & Validation

Tested ApplicationsIHC, IP, WB
Dilution rangeWB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-RALY antibody A302-069A.; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ReactivityHuman, Mouse
Application Notes
Format: Whole IgG

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenBetween 256 and C-terminus
TargetRALY
PurificationAntigen Affinity Purified
ConjugationUnconjugated

Storage & Handling

Storage2 - 8°C
Form/AppearanceLiquid
Buffer/PreservativesTris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration200 µg/ml
DisclaimerFor research use only

Alternative Names

autoantigen p542; heterogeneous nuclear ribonucleoprotein C-like 2; hnRNP associated with lethal yellow protein homolog; hnRNP core protein C-like 2; HNRPCL2; P542; RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog); RNA-binding protein (autoantigenic); RNA-binding protein (autoantigenic, hnRNP-associated with lethal yellow); RNA-binding protein RalY

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Rabbit RALY Antibody

Detection of human RALY by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, HEK293T, Hep-G2, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RALY antibody (orb1527278) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Rabbit RALY Antibody

Detection of human RALY by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from U2OS cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RALY antibody (orb1527278) used for IP at 6 µg per reaction. RALY was also immunoprecipitated by a previous lot of this antibody (orb1527278) and a second antibody against a different epitope of RALY.

Rabbit RALY Antibody

Detection of human RALY by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RALY (orb1527278) used at a dilution of 1: 1, 000 (0.2 µg/ml).

Rabbit RALY Antibody

Detection of mouse RALY by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RALY (orb1527278) used at a dilution of 1: 1, 000 (0.2 µg/ml).

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IP
Immunoprecipitation
View Protocol

Rabbit RALY Antibody (orb1527278)

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Available Sizes

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10 μl
¥ 3,120.00
100 μl
¥ 8,190.00
DispatchUsually dispatched within 5-10 working days
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