Rabbit SPECC1 Antibody

SKU: orb1526672

Description

SPECC1 Antibody

Research Area

Cancer Biology

Images & Validation

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Item 1 of 1

Tested Applications	IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 450 and 500
Target	SPECC1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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cytokinesis and spindle organization B; cytospin-B; CYTSB; HCMOGT1; HCMOGT-1; NSP; NSP5; nuclear structure protein 5; sperm antigen HCMOGT-1; Sperm antigen with calponin homology and coiled-coil domains 1; sperm antigen with calponin-like and coiled coil domains 1; structure protein NSP5a3a; structure protein NSP5a3b; structure protein NSP5b3a; structure protein NSP5b3b

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Detection of human SPECC1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPECC1 antibody orb1526672 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. SPECC1 was also immunoprecipitated by rabbit anti-SPECC1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Quick Database Links

Gene Symbol

SPECC1

UniProt

Q5M775

RefSeq (Protein):NP_001028725.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001028725.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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