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RBM8A/Y14 Antibody
SKU: orb1151583
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 3
| Tested Applications | FACS, IF, WB |
|---|---|
| Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | PCRP-RBM8A-1B4 |
| Immunogen | Recombinant full-length human RBM8A protein was used as the immunogen for the RBM8A/Y14 antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Immunofluorescent staining of PFA-fixed human HeLa cells using RBM8A/Y14 antibody (green, clone PCRP-RBM8A-1B4) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with RBM8A/Y14 antibody (blue, clone PCRP-RBM8A-1B4), and unstained cells (red).
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using RBM8A/Y14 antibody (clone PCRP-RBM8A-1B4). These results demonstrate the foremost specificity of the PCRP-RBM8A-1B4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
RBM8A/Y14 Antibody (orb1151583)
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