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RGS14 Antibody
SKU: orb18750
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, FC, IF |
|---|---|
| Dilution range | ELISA: 1:16000, WB: 0.5-2 μg/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | RGS14 |
| Molecular Weight | 61.4 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti RGS14 antibody, anti regulator of G-protein signalling 14 antibody, anti regulation of G protein signaling 14 antibody
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Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Protocol Information
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
RGS14 Antibody (orb18750)
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