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S100-A8 / MRP8 Polyclonal Antibody
SKU: orb1417584
Description
Research Area
Apoptotic, Autophagic, Cancer, Epigenetics, Immunology, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IF |
|---|---|
| Reactivity | Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Disclaimer | For research use only |
Alternative Names
−Protein S100-A8, Calgranulin-A, Chemotactic cytokine CP-10, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8, MRP-8, p8, Pro-inflammatory S100 cytokine, S100 calcium-binding protein A8, S100a8, Caga, Mrp8
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S100-A8 / MRP8 Polyclonal Antibody [orb1089039]
FC
Mouse, Rat
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl
Quality Guarantee
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Blank control: K562.Primary Antibody (green line): Rabbit Anti-S100-A8 antibody.Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE. Dilution: 1 µg /test.Protocol. The cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20, 000 events was performed.
Blank control: Mouse spleen.Primary Antibody (green line): Rabbit Anti-S100-A8 antibody.Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488. Dilution: 1 µg /test.Protocol. The cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20, 000 events was performed.
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Protocol Information
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
S100-A8 / MRP8 Polyclonal Antibody (orb1417584)
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