S100A12 Antibody / CGRP

SKU: orb1824399

Description

S100A12 (CGRP) is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its pro-inflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration.

Images & Validation

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Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the S100A12 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	S100A12/4514
Immunogen	A recombinant partial protein sequence (within amino acids 1-95) from the human protein was used as the immunogen for the S100A12 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human placental tissue with S100A12 antibody (clone S100A12/4514). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free S100A12 antibody (clone S100A12/4514) as confirmation of integrity and purity.

Quick Database Links

UniProt

P80511

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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