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ExoN Antibody

SKU: orb1239077

Description

ExoN Antibody

Images & Validation

Tested ApplicationsELISA, IHC
ReactivityVirus

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenAnti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody (orb1239077) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP14 (ExoN) protein. The immunogen is located between 120-170 amino acids of SARS-CoV-2 (COVID-19) NSP14 (ExoN).
TargetExoN
PurificationSARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is affinity chromatography purified via peptide column.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration1 mg/mL
DisclaimerFor research use only

Alternative Names

Proofreading exoribonuclease, Guanine-N7 methyltransferase, Non-structural protein 14, NSP14.

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

ExoN Antibody

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP14 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) NSP14 antibody (orb1239077, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP14 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ExoN Antibody

ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody, orb1239077. A direct ELISA was performed using immunogen peptide (orb1231678) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ExoN Antibody (orb1239077)

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Available Sizes

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0.02 mg
¥ 3,770.00
0.1 mg
¥ 8,710.00
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