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Description
Research Area
Infectious Disease & Virology, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IF, WB |
|---|---|
| Dilution Range | IF: 1:25, WB: 1:1000, FC: 1:25 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,κ |
| Immunogen | This SP1 antibody is generated from a mouse immunized with a recombination protein from the human SP1. |
| Target | SP1 |
| Molecular Weight | 80693 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Transcription factor Sp1, SP1, TSFP1
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Flow cytometric analysis of Hela cells using SP1 Antibody (green) compared to an isotype control of mouse IgG1 (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from Hela, Ramos cell line (from left to right), using SP1 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Fluorescent image of Hela cells stained with SP1 Antibody. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Quick Database Links
Gene Symbol
SP1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
SP1 Antibody (orb1788414)
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