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T7 tag

SKU: orb1787854

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

Tested ApplicationsWB
Dilution RangeWB: 1:1000

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenThis antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide of T7 tag.
TargetThis antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide of T7 tag.
Molecular Weight45-50KD
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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T7 tag

All lanes: Anti-T7 tag Antibody at 1:1000 dilution. Lane 1: 293T/17 transfected with 12tag lysate (1ug). Lane 2: Non-transfected 293T/17 lysate (1ug). Lane 3: 12tag recombinant protein lysate (0. 1ug). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

T7 tag (orb1787854)

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