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TB5

SKU: orb1304016

Description

TB5

Research Area

Metabolism Research, Neuroscience

Images & Validation

Key Properties

CAS Number948841-07-4
MW336.25
Purity99.82%
FormulaC15H14BrNOS
SMILESCN(C)c1ccc(\C=C\C(=O)c2ccc(Br)s2)cc1
TargetMAO,Monoamine Oxidase
SolubilityDMSO:55 mg/mL (163.57 mM)

Bioactivity

Target IC50
MAO-B (human):0.1 ± .01 μM. (Ki)
In Vivo
Compounds are initially dissolved in DMSO (5 mg/mL), then further diluted with a PBS/EtOH mixture (70:30). To elucidat the critical interactions dictating selectivity and potency, kinetic Analyses of TB5 and TB8 are conducted. Lineweaver–Burk plots, a total of five, are created to compar the catalytic rates of MAO-B and MAO-A at various substratee concentration (0.1-1 μM) in bot the absence and presence of TB5 and TB8 at differing concentration the initial plot reveal the baseline enzyme activity without inhibitors, whil the subsequent four plots sho the impact of varying concentration of TB5 and TB.
In Vitro
TB5 and TB8 interact competitively wit the catalytic sites of hMAO-B and hMAO-A. TB5 show the highest inhibitory activity and selectivity toward hMAO-B, with Ki and SI Values of 0.1 ± .01 μM and 13.18, respectively Inhibition by TB5 (hMAO-B) and TB8 (hMAO-A) is fully reversible after 24 hours of dialysis. Cytotoxicity studies reveal TB5 is non-toxic at 5 and 25 μM, with cell viabilities of 95.75% and 84.59%, respectively.
Cell Research
In vitro cytotoxicity of brominated thiophene chalcones and s andard MAO inhibitors are tested in human HepG2 hepatic cancer cells at three different concentration (1, 5 and 25 μM.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
DisclaimerFor research use only

Alternative Names

MonoamineOxidase, Monoamine Oxidase, inhibit, Inhibitor, hMAO-B, MAO, TB 5, TB5, TB-5

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Key Properties

No computed properties available.

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TB5 (orb1304016)

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% DMSO +
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% Tween 80 +
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Available Sizes

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1 ml x 10 mM (in DMSO)
¥ 1,170.00
5 mg
¥ 1,170.00
10 mg
¥ 1,430.00
25 mg
¥ 2,210.00
50 mg
¥ 3,120.00
100 mg
¥ 4,680.00
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