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Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:10~50 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Hamster, Other, Porcine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b,κ |
| Immunogen | TBB5 recombinant protein is used to produce this monoclonal antibody. Antigen Region: 1-219 aa. |
| Target | TUBB |
| Molecular Weight | 49671 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TUBB; TUBB5; Tubulin beta chain; Tubulin beta-5 chain
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Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from mouse brain tissue, Hela, Y79, CEM, rat PC-12 cell line (from left to right), using TBB5 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescent analysis of A549 cells, using TBB5 Antibody. Diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_821133.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
TBB5 Antibody (orb1788437)
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