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4-Di-1-ASP

SKU: orb3027083

Description

4-Di-1-ASP is a positively charged fluorescent dye used to label glioma cells in live brain tissue and can also be used to observe mitochondria in live cells, with λex/λem = 475/606 nm.

Research Area

Metabolism Research

Images & Validation

Key Properties

CAS Number959-81-9
MW366.24
Purity99.80%
FormulaC16H19IN2
SMILES[I-].C(=CC1=CC=[N+](C=C1)C)C2=CC=C(C=C2)N(C)C
TargetMitochondrial Metabolism
SolubilityDMSO:40 mg/mL (109.22 mM)

Bioactivity

In Vitro
Instructions (The following is a recommended protocol; actual procedures may be adjusted according to specific experimental needs): Dissolve the 4-Di-1-ASP in distilled water to prepare a 2 mM stock solution. Place the cell sections in a 35°C incubator, add the working dye solution to achieve a final concentration of 1 μM, and incubate for 10 minutes. After staining, mount the sections on a microscope slide, cover with a coverslip, and observe using a confocal fluorescence microscope. The optimal excitation and emission wavelengths are 475 nm and 606 nm, respectively.

Storage & Handling

Storagekeep away from moisture,keep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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Protocol Information

4-Di-1-ASP (orb3027083)

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(Recommended: An additional animal making an allowance for loss during the experiment)

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100 mg
¥ 1,040.00
200 mg
¥ 1,300.00
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