5-Azidomethyl-2'-deoxyuridine (5-AmdU)

5-AmdU (5-Azidomethyl-2'-deoxyuridine) can be used as a replace-ment for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-AmdU is cell permeable and incorporates into peplicating DNA instead of its natural analog thymidine. The resulting azide-functionalized DNA can subsequently be detected via Cu(I)-catalyzed (CuAAC) or Cu(I)-free Click Chemistry that offers the choice to: introduce a Biotin group for subsequent purification tasks (via Alkynes of Biotin or DBCO-functionalized Biotin, respectivly); introduce a fluorescent group for subsequent microscopic imaging (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectivly).