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5T4 Recombinant Rabbit Monoclonal Antibody
SKU: orb1816795
Description
Research Area
Cancer Research, Developmental Biology, Stem Cell Research
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 23B1 |
| Immunogen | KLH conjugated synthetic peptide derived from human TPBG/5T4 |
| Target | Tpbg |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−5T4; 5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; 5T4AG; M6P1; TPBG; TPBG_HUMAN; Trophoblast glycoprotein.
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WB
Human, Mouse
Rabbit
Monoclonal
Unconjugated
30 μl, 50 μl, 100 μl, 200 μl
Quality Guarantee
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Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: MCF-7, 2: JAR, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 46 kDa, Observed MW: 70-80/85 kDa.
IP of MCF-7 cells extracts, IP ab incubation condition: orb1816795, 4°C, overnight, 1:100 dilution, WB primary ab incubation condition: orb1816795, room temperature 2h, 1:1000 dilution, Secondary ab: Anti-Rabbit IgG for IP (HRP), Blocking buffer and Concentration: 5% , NFDM/TBST, Diluting buffer and Concentration: 5% , NFDM/TBST, 1: 5% Input, 2: IP with orb1816795, 3: IP with Rabbit monoclonal IgG Isotype, Observed MW: 70-80/85 kDa, Exposure time: 120 s.
Tissue: Human placenta, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1816795.
Quick Database Links
Gene Symbol
Tpbg
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
5T4 Recombinant Rabbit Monoclonal Antibody (orb1816795)
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