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ABCB4 Antibody (Center)
SKU: orb1930247
Description
Research Area
Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | WB - 1:500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 141523 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Quality Guarantee
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
ABCB4-S639 western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the ABCB4 antibody detected ABCB4 protein (arrow).
Anti-hABCB4-S639 at 1:2000 dilution + Human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 142 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-hABCB4-S639 at 1:500 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 142 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ABCB4 Antibody (Center) (orb1930247)
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