Acetyl-Histone H3 (Lys27) Rabbit mAb

SKU: orb1564022

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Description

Research Area

Epigenetics & Chromatin

Images & Validation

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Item 1 of 3

Tested Applications	ChIP, ICC, IF, IHC-Fr, IHC-P, IP, WB
Dilution range	WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	IgG
Clone No.	R08-4A0
Immunogen	A synthetic acetyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
Target	H3C1
Molecular Weight	Calculated MW: 15 kDa; Observed MW: 15 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin.
Concentration	0.3 mg/ml
Disclaimer	For research use only

Alternative Names

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H3K27ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J

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Western blot analysis of Histone H3 (acetylLys27) in Hela, A549, HL-60 lysates using Acetyl-Histone H3 (Lys27) antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Histone H3 (acetylLys27) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of Histone H3 (acetylLys27) (green) in hela using Histone H3 (acetylLys27) antibody, and DAPI (blue).