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Acid Phosphatase Antibody Biotin Conjugated

SKU: orb344938

Description

Acid Phosphatase antibody (Biotin)

Images & Validation

Tested ApplicationsDOT
Dilution rangeWB: User Optimized
ReactivityPlant
Application Notes
Anti-Acid Phosphatase Biotin Conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Acid Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:32,000 of the reconstitution concentration is suggested for this product.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenAcid Phosphatase [Potato]
PurityAcid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato]. Cross reactivity against Acid Phosphatase from other sources is unknown.
ConjugationBiotin

Storage & Handling

StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Form/AppearanceLiquid (sterile filtered)
Buffer/Preservatives0.01% (w/v) Sodium Azide
Concentration1.0 mg/mL
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

rabbit anti-Acid Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Acid Phosphatase Antibody, Acid phosphatase 1 soluble antibody, PAP3, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody

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Acid Phosphatase Antibody Biotin Conjugated

Dot Blot results of Rabbit Anti-Acid Phosphatase Biotin Conjugated. Antigen: Acid Phosphatase. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Acid Phosphatase Biotin 10 µg/ml for 1hr at RT. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

DOT
Dot Blot
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Acid Phosphatase Antibody Biotin Conjugated (orb344938)

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