Acid Phosphatase Antibody Peroxidase Conjugated

SKU: orb1463567

Description

Acid Phosphatase antibody (HRP)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, ELISA, WB
Dilution range	ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
Reactivity	Plant
Application Notes	Anti-Acid Phosphatase Peroxidase Conjugated Antibody has been tested by dot blot and western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Acid Phosphatase [Potato]
Purity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato]. Cross reactivity against Acid Phosphatase from other tissues and species may occur but have not been specifically determined.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Disclaimer	For research use only

Alternative Names

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rabbit anti-Acid Phosphatase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Acid Phosphatase Antibody, Acid phosphatase 1 soluble antibody, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody

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Acid Phosphatase Antibody Peroxidase Conjugated

Western Blot of Acid Phosphatase (Potato) Antibody. Lane 1: Acid Phosphatase (Potato). Load: 50 ng per lane. Primary antibody: Acid Phosphatase Antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 49 kDa/42-45kDa for Acid Phosphatase (Potato) Protein. Other band(s): Acid Phosphatase monomer fragmentation.

Quick Database Links

UniProt

Q6J5M8

RefSeq:AAT37528.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	AAT37528.1

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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