Acriflavine

SKU: orb1706392

Description

Acriflavine is a fluorescent dye and papain-like protease (PLpro) inhibitor with antimicrobial and anticancer properties. It is utilized in research for labeling high molecular weight RNA and studying β-coronaviruses like SARS-CoV-2, as well as in investigations of leukemia in both in vitro and in vivo models.

Research Area

Epigenetics & Chromatin, Infectious Disease & Virology, Metabolism Research, Pharmacology & Drug Discovery

Images & Validation

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Key Properties

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CAS Number	8048-52-0
MW	468.98
Formula	C₂₇H₂₅ClN₆
SMILES	[Cl-].Nc1ccc2cc3ccc(N)cc3nc2c1.C[n+]1c2cc(N)ccc2cc2ccc(N)cc12
Target	Monocarboxylate transporter,SARS-CoV,HIF/HIF Prolyl-Hydroxylase,Antibacterial,Antifungal
Solubility	H2O:20 mg/mL (42.65 mM)

Bioactivity

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In Vivo	Nude mice bearing xenografts of A549 cells were intraperitoneally injected with 2 mg/kg ACF daily for six weeks. The results indicate that treatment with ACF leads to a reduction in tumor size. Read more...
In Vitro	A novel candidate drug, Acriflavine, exhibits significant dose-dependent inhibition of A549 cell viability at low concentrations (1, 2, 5, and 10 μM).
Cell Research	1. RNA marker Experimental steps: 1. Prepare solution: Dissolve Acriflavine in an appropriate solvent, such as DMSO or water, and the concentration is adjusted according to the experimental needs. 2. Label RNA: Add solution (10-100uM) to the RNA sample and incubate for a certain period of time (usually 30 minutes to 1 hour). 3. Fluorescence measurement: Use a fluorescence spectrophotometer or fluorescence microscope to detect the fluorescence signal of RNA and evaluate labeling efficiency. 2. Antibacterial experiments Experimental steps: 1. Dissolve Acriflavine: Dissolve Acriflavine in an appropriate solvent (usually in PBS or water). 2. Treat bacteria: Contact Acriflavine solution at different concentrations with the bacteria to be tested and incubate for a certain period of time. 3. Detection of inhibitory effect: Measure the antibacterial effect by bacterial growth curve or plate counting method. III. Anti-cancer research Experimental steps: 1. Dissolve Acriflavine: Dissolve Acriflavine in an appropriate solvent (such as DMSO). 2. Treat tumor cells: Add different concentrations of Acriflavine solution to cultured tumor cells and incubate for a certain period of time. 3. Cell proliferation detection: Use MTT method, cloning experiments or other cell proliferation detection methods to evaluate the anti-cancer activity of Acriflavine. IV. SARS-CoV-2 inhibition experiment Experimental steps: 1. Dissolve Acriflavine: Prepare Acriflavine solution in an appropriate solvent. 2. Contact with the virus: Add the Acriflavine solution to the virus culture system and contact with SARS-CoV-2 at different concentrations. 3. Viral inhibition detection: Viral replication level is detected by RT-PCR, virus titer experiments or immunofluorescence analysis. 5. Papain-like protease inhibition experiment: Experimental steps: 1. Prepare Acriflavine solution: Dissolve Acriflavine in an appropriate solvent and adjust the concentration. 2. Reaction with enzymes: Add Acriflavine to the reaction system of papain-like protease and incubate. 3. Measuring enzyme activity: Evaluate the inhibitory effect of Acriflavine on enzyme activity by enzyme activity detection methods, such as substrate cleavage experiments. Read more...
Animal Research	CML mice are treated daily with acriflavine (8 mg/kg) or PBS via intraperitoneal injection, for 10 days starting from day 7 after bone marrow transplantation . Read more...

Storage & Handling

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Storage	keep away from direct sunlight,keep away from moisture \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Antibacterial, Acriflavine, Acriflavinium chloride 3,6-Acridinediamine mix, SARS-CoV-2, SARSCoV

Quality Guarantee

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Compound Identifiers

PubChem CID

443101 ↗

Key Properties

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No computed properties available.

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Acriflavine (orb1706392)

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Applications:	ELISA, WB
Reactivity:	E. coli
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, WB
Reactivity:	E. coli
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, WB
Reactivity:	E. coli
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, WB
Reactivity:	E. coli
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Purity:	99.69%
CAS Number:	8063-24-9
MW:	259.73
Formula:	C₁₄H₁₄ClN₃

Acriflavine

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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