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Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB: 1:1000, WB: 1:1000, FC: 1:10~50 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This ADA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the C-terminal region of human ADA. Antigen Region: 287-314 aa. |
| Target | ADA |
| Molecular Weight | 40764 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ADA; ADA1; Adenosine deaminase; Adenosine aminohydrolase
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ADA Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from Jurkat cell line, mouse stomach tissue lysate (from left to right), using ADA Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
All lanes: Anti-ADA Antibody (C-term) at 1:1000 dilution. Lane 1: MOLT-4 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000013.2 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ADA Antibody (C-term) (orb1788248)
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