Vazyme - Add&Read Human Fc Kit (broad range)

Human Fc Kit (broad range) can be used to detect the concentration of Human IgG or hFc-fusion protein in cell supernatant or after purification by high throughput. There is an antibody strain in the reagent kit that recognizes the Fc region of Human IgG and labeled with the fluorescent donor Eu (Anti-human IgG-Eu); There is also Human IgG, labeled with fluorescent receptor A2 (Human IgG-A2). In the solution, Anti-human IgG-Eu binds to Human IgG-A2, and 320 nm light excites the fluorescent donor Eu,, and the Eu donor emits 620 nm light, which emits 620 nm light, which excites the fluorescent receptor A2, which emits 665 nm light, which is a phenomenon of Fluorescence Resonance Energy Transfer (FRET). When adding Human IgG or hFc-fusion protein to the solution, it will compete to bind Anti-human IgG-Eu and destroy FRET phenomenon. The concentration of Human IgG or hFc-fusion protein to be measured is inversely proportional to the signal value of FRET (Light Intensity Ratio of 665 nm/620 nm).