Vazyme - Add&Read Human IgG Kit

Human IgG Kit is designed for detecting the concentration of Human IgG in cell supernatants or after purification. The kit contains two antibodies that recognize Human IgG, which are: an antibody that recognizes the Fc region, labeled with the luminescent donor Europium (Anti-hFc-Eu); and an antibody that recognizes the Fab region, labeled with the luminescent acceptor A2 (Anti-hFab-A2). When Anti-hFc-Eu and Anti-hFab-A2 are in close proximity (binding occurs), Fluorescence Resonance Energy Transfer (FRET) can take place. Using light at 320 nm to excite the luminescent donor Eu, Eu emits light at 620 nm, which in turn excites the luminescent acceptor A2, and A2 emits light at 665 nm. The concentration of Human IgG to be tested is directly proportional to the FRET signal value (the ratio of the light intensity at 665 nm to 620 nm).