Vazyme - Add&Read Mouse Fc Kit

The Mouse Fc Kit can be used for high-throughput detection of the concentration for Mouse IgG or mFc fusion protein in cell supernatant or after purification. There is an antibody strain in the reagent kit that recognizes the Fc region of Mouse IgG and labels the fluorescent donor Eu (Anti-Mouse IgG-Eu); There is also a Mouse IgG labeled with fluorescent receptor A2 (Mouse IgG-A2). In the solution, Anti-Mouse IgG-Eu binds to Mouse lgG-A2, and 320 nm light excites the fluorescent donor Eu, which emits 620 nm light, which excites the fluorescent receptor A2, which emits 665 nm light, which is a phenomenon of Fluorescence Resonance Energy Transfer (FRET) . When adding Mouse IgG or mFc-fusion protein to the solution, it will compete to bind Anti-Mouse IgG-Eu and destroy FRET phenomenon. The concentration of Mouse IgG or mFc-fusion protein to be measured is inversely proportional to the signal value of FRET (light intensity ratio of 665 nm/620 nm) .