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AES-350

SKU: orb1298420

Description

AES-350 is a potent, orally bioavailable HDAC6 inhibitor with high selectivity. It induces apoptosis in acute myeloid leukemia (AML) cells in vitro and shows potential for in vivo research, making it a valuable tool for oncology and epigenetics studies.

Research Area

Cell Biology, Epigenetics & Chromatin, Molecular Biology

Images & Validation

Key Properties

CAS Number847249-57-4
MW312.36
Purity99.20% (May vary between batches)
FormulaC18H20N2O3
SMILESCC(C)(C)c1ccc(cc1)C(=O)Nc1ccc(cc1)C(=O)NO
TargetHDAC,Apoptosis
Solubility10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (12.81 mM);DMSO:100 mg/mL (320.14 mM)

Bioactivity

Target IC50
HDAC6:24.4 nM|HDAC3:187 nM|HDAC11:245 nM
In Vitro
AES-350 (0.25-4 μM; 18 hours) induces MV4-11 cells apoptosis in a dose-dependent manner. The late apoptosis ratios are 8.74%, 11.7%,16.08%, 30.97%, and 38.48%, respectively at 0.25 μM-4 μM. AES-350 has submicromolar activity (IC50=0.58±0.13 μM) against MV4-11 cells than to that of vorinostat (IC50=0.31±0.061 μM). AES-350 is more ligand efficient and exemplifies a large therapeutic index (IC50>30 μM in noncancerous MRC-9 cells). AES-350 is also shown to be effective in AML-3 (acute myeloid leukemia) cells (IC50=0.73 ± 0.12 μM). An ELISA is performed using HeLa cervical cancer cell lysates, and HeLa cells highly express HDAC6 and are sensitive to AES-350. Correspondingly, ELISA assays depicted a dose-dependent increase in HDAC6 inhibition (IC50=0.58±0.13 μM), Western blot analysis shows that AES-350 (0.1-10 μM) induces a dose-dependent increase in acetylated α-tubulin (Ac-α-tubulin), a substrate of HDAC.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Histone deacetylases, Inhibitor, HDAC, HDAC11, HDAC3, HDAC6, MV4-11, inhibit, Apoptosis, AML, acute myeloid leukemia, AES 350, AES350, AES-350

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Quality Guarantee

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Key Properties

No computed properties available.

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Protocol Information

AES-350 (orb1298420)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

1 mg
¥ 1,950.00
5 mg
¥ 4,030.00
10 mg
¥ 5,460.00
25 mg
¥ 9,490.00
50 mg
¥ 13,000.00
100 mg
¥ 17,810.00
DispatchUsually dispatched within 3-5 working days
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