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AFP Recombinant Rabbit Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-5000, IHC-P=1:100-500, IF=1:100-500, IHC-F=1:100-500 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | A5H7 |
| Immunogen | A synthesized peptide derived from human alpha Fetoprotein (1-609/609aa) |
| Target | AFP |
| Molecular Weight | 65 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized or Liquid |
| Buffer/Preservatives | PBS, Glycerol, rAlbumin. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody [orb1410286]
IHC
Human
Rabbit
Monoclonal
Unconjugated
100 μg (without BSA and Azide), 100 μg, 20 μg

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(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AFP Monoclonal Antibody, Unconjugated (orb2563493) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AFP Monoclonal Antibody, Unconjugated (orb2563493) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AFP Monoclonal Antibody, Unconjugated (orb2563493) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
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Protocol Information
AFP Recombinant Rabbit Monoclonal Antibody (orb2563493)
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