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AHA1 Antibody
SKU: orb1822460
Featured
Description
Research Area
Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, ICC, IF, IHC, IP, WB |
|---|---|
| Dilution range | WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP ( 1:1000); optimal dilutions for assays should be determined by the user. |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rat |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2a Kappa |
| Clone No. | 25F2.D10 |
| Immunogen | Mouse Aha1 |
| Target | AHA1 |
| Molecular Weight | 38kDa |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2 *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−AHSA1, AHA1, HSPC322, p38, C14orf3, LOC10598, Activator of Hsp90 ATPase activity 1, Activator of 90 kDa heat shock protein ATPase homolog 1
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Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10. Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).
Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10. Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000.
Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.
Western Blot analysis of Human Cell lysates showing detection of Aha1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_666148.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
AHA1 Antibody (orb1822460)
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