AIRE Rabbit pAb Discontinued

SKU: orb135062

This product can be custom made

Description

Rabbit polyclonal antibody to AIRE

Research Area

Epigenetics

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, WB
Dilution Range	WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 30-130 of human AIRE (NP_000374.1).
Molecular Weight	55kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti APS1 antibody, anti APSI antibody, anti PGA1 antibody, anti AIRE1 antibody, anti APECED antibody

Quality Guarantee

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Immunofluorescence analysis of C6 cells using AIRE Rabbit pAb (orb135062) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using AIRE Rabbit pAb (orb135062) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using AIRE Rabbit pAb (orb135062) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using AIRE antibody (orb135062) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.