You have no items in your shopping cart.
AKT Mouse Monoclonal Antibody
SKU: orb526606
Featured
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, WB |
|---|---|
| Dilution range | WB=1:1000-5000, ICC/IF=1:100-500 |
| Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | 10D8 |
| Immunogen | KLH conjugated synthetic peptide derived from human AKT (420-479/479aa) |
| Target | AKT1 |
| Molecular Weight | 56 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−AKT 1; AKT; AKT1; AKT-1; AKT1_HUMAN; C AKT; cAKT; MGC9965; MGC99656; Oncogene AKT1; PKB; PKB alpha; PKB-ALPHA; PRKBA; Protein Kinase B alpha; Protein kinase B; Proto-oncogene c-Akt; RAC alpha; RAC alpha serine/threonine protein kinase; RAC; RAC PK alpha; Rac protein kinase alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; v akt murine thymoma viral oncogene homolog 1; vAKT Murine Thymoma Viral Oncogene Homolog 1.
Similar Products
−- Item 1 of 6
Phospho-AKT (Ser473) Mouse Monoclonal Antibody [orb506119]
IF, IHC-Fr, IHC-P
Bovine, Canine, Porcine, Rabbit, Sheep
Human, Mouse, Rat
Mouse
Monoclonal
Unconjugated
200 μg, 100 μl, 200 μl, 50 μl - Item 1 of 7
- Item 1 of 6
- Item 1 of 3
- Item 1 of 3
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Sample: MCF-7 (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-AKT (orb526606) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.
Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MAKT) Monoclonal Antibody, Unconjugated (orb526606) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG-CY3 antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
AKT1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
AKT Mouse Monoclonal Antibody (orb526606)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days