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Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IP, WB |
|---|---|
| Dilution Range | ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000 |
| Reactivity | Yeast |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Alcohol Dehydrogenase [Yeast] |
| Target | ADH1 |
| Purity | Anti-Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, ADH, ADC
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Western Blot of Alcohol Dehydrogenase Antibody. Lane 1: Alcohol Dehydrogenase. Load: 50 ng per lane. Primary antibody: Alcohol Dehydrogenase Antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase Conjugated Rabbit secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 Blocking Buffer for 30 min at RT. Predicted: 37kDa/Observed size: 45kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
ADH1 Antibody (orb344798)
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