Alginate Antibody (APC)

SKU: orb181900

Description

Mouse monoclonal antibody against Alginate, conjugated to APC.

Images & Validation

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Tested Applications	IHC, WB
Dilution range	WB (1:500)
Reactivity	All
Application Notes	1 µg/ml of SMC-209 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1 Kappa
Clone No.	3G4-1F5
Immunogen	Sodium Alginate conjugated to KLH
Target	Alginate
Purification	Protein G Purified
Conjugation	APC

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Alginate, a naturally derived polysaccharide from brown seaweed, is widely used in biomedical research due to its excellent biocompatibility, low immunogenicity, and ability to form hydrogels through ionic crosslinking with divalent cations such as calcium (Ca²⁺). In neuroscience and neurodegenerative disease research, alginate-based systems are gaining traction as advanced platforms for cell encapsulation, controlled drug delivery, and 3D neural tissue modeling. One of alginate’s most impactful applications is in the formation of microspheres and scaffolds that enable sustained, localized delivery of neuroprotective proteins and therapeutic agents. This controlled release capability is critical for maintaining therapeutic concentrations in the brain microenvironment, particularly in diseases such as Alzheimer’s, Parkinson’s, and amyotrophic lateral sclerosis (ALS), where targeted intervention is essential. Moreover, alginate hydrogels support the encapsulation and transplantation of neural stem cells and glial cells, preserving cell viability and function while minimizing immune rejection. These properties make alginate an ideal candidate for developing bioengineered neural tissues, in vitro brain models, and regenerative therapies aimed at restoring damaged neural circuits. As research advances, alginate continues to emerge as a key biomaterial in the design of next-generation neurotherapeutic strategies, offering promising avenues for both fundamental neuroscience and translational applications in neurodegenerative disease treatment.

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Western Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 3G4-1F5. Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa.

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Gene Symbol

Alginate

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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