Alginate Antibody (HRP)

SKU: orb181885

Description

Mouse monoclonal antibody against Alginate, conjugated to HRP.

Images & Validation

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Tested Applications	IHC, WB
Dilution range	WB (1:500), IHC (1:200)
Reactivity	All
Application Notes	1 µg/ml of SMC-208 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1 Kappa
Clone No.	4B10-1C5
Immunogen	Sodium Alginate conjugated to KLH
Target	Alginate
Purification	Protein G Purified
Conjugation	HRP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Alginate, Sodium alginate, Calcium alginate, Alginic acid, Algin, Alginates, Alginic polysaccharide, Alginic acid sodium salt, Alginic acid calcium salt, Seaweed polysaccharide, Brown algae polysaccharide

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Immunohistochemistry analysis using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5. Tissue: Burned Skin. Species: Human. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody at 1:200.

Western Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5. Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa.

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Gene Symbol

Alginate

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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