Anti-AIF1 [GT1-mAb1]

Flow cytometry using anti-AIF1 antibody GT1-mAb1. Paraformaldehyde fixed human peripheral blood monocytes permeabilized with 0.5% Triton were stained with the isotype control antibody (black line) or the rabbit IgG version of GT1-mAb1 (orb2645766, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of U937 cells with anti-AIF1 antibody GT1-mAb1. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of GT1-mAb1 (orb2645766) (10 ug/ml) for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645766, DAPI, merged channels and an isotype control. The isotype control was an anti-fluorescein antibody followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-AIF1 antibody GT1-mAb1. RAW264.7 (A) (2 µg/ml), U937 (B) (2 µg/ml) cells lysate, and mouse brain (C) (2 µg/ml) tissue lysate (35 µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of GT1-mAb1 (orb2645766) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.