alpha-Fetoprotein Antibody (Biotin)

SKU: orb1480515

Description

Mouse monoclonal antibody against alpha-Fetoprotein conjugated to Biotin

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, ICC, IP, WB
Reactivity	Human
Application Notes	ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions. Read more...

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	AFP-01
Immunogen	purified alpha-Fetoprotein
Target	alpha-Fetoprotein
Purification	Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Conjugation	Biotin

Storage & Handling

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Storage	Store at 2-8°C. Do not freeze.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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AFP, FETA, HPAFP

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ELISA analysis of alpha-fetoprotein (AFP) using biotin-conjugated detection antibody AFP-01. Different alpha-fetoprotein concentrations (0 - 5 μg/ml) and different detection antibody concentrations (0.016 - 10 μg/ml) were tested.

Quick Database Links

Gene Symbol

alpha-Fetoprotein

UniProt

P02771

NCBI Gene ID

174

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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