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Description
Research Area
Apoptotic, Cancer, Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1-5000-1-10000, WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500 |
| Reactivity | Bovine, Human, Mouse, Porcine, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BAX. The exact sequence is proprietary. |
| Target | BAX |
| Molecular Weight | 21 kDa |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4
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Immunofluorescent analysis of BAX staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of paraffin-embedded rat kidney using BAX Antibody orb213599 with dilution at 1:100.
Immunohistochemical analysis of paraffin-embedded human breast cancer using BAX Antibody orb213599 with dilution at 1:100.
Western blot analysis of mouse brain (1), rat lung (2) using BAX Antibody orb213599 with dilution at 1:1000.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
BAX Rabbit Polyclonal Antibody (orb213599)
- 5.0
Based on 2 reviews
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- 5 stars
Probing lysates with the Biorbyt Bax antibody sample revealed a single, specific band at ~21 kDa, consistent with the predicted molecular weight and free of significant smearing. The blot displayed a clean background and negligible nonspecific reactivity. Upon chemiluminescent detection, the bands were sharp and well-defined, with reliable experimental reproducibility, confirming the antibody's suitability for detecting apoptosis-related proteins.
- 5 stars
Western blot analysis using the biorbyt Bax antibody detected a single, distinct band at approximately 21 kDa, consistent with the predicted molecular weight. The assay exhibited minimal nonspecific binding, a clean background, a high signal-to-noise ratio, and excellent band contrast.
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