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Anti-Bet v 1 [BV16]
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Plant |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | BV16 |
| Immunogen | Bet v 1-A, a major allergen of the European white birch. |
| Target | Bet v 1 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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![Anti-Bet v 1 [BV16]](/images/pub/media/catalog/product/NewWebsite/35/orb613896_1.png)
Binding curve of anti-Bet v 1 antibody BV16 (orb613897) to Bet v 1 ELISA Plate coated with Bet v 1-A at a concentration of 2 µg/ml. A 3-fold serial dilution from 10000 to 0.1 ng/ml was performed using orb613897. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more, as also observed for orb613899. This is suggestive of two binding sites on Bet v 1-A for which orb613897 has differing affinities.
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Protocol Information
Anti-Bet v 1 [BV16] (orb613896)
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