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CARD12 Antibody
SKU: orb412596
Featured
Description
Research Area
Apoptotic, Cancer, Epigenetics, Immunology, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human CARD12 |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−CARD12; CLAN; CLAN1; IPAF; NLR family CARD domain-containing protein 4; CARD, LRR, and NACHT-containing protein; Clan protein; Caspase recruitment domain-containing protein 12; Ice protease-activating factor; Ipaf
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Western blot analysis of CARD12 expression in MCF7 (A), mouse heart (B), rat kidney (C) whole cell lysates. (Predicted band size: 116 kD; Observed band size: 110 kD)
Immunofluorescent analysis of CARD12 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
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Zhang, Xuling et al. Effects of lysophosphatidic acid receptor 5 on NLRC4 inflammasome in brain tissues of transient cerebral ischemia/reperfusion rat Hum Exp Toxicol, 41, (2022)
Applications
WB
Reactivity
Rat
CARD12 Antibody (orb412596)
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